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- Keywords recombinant proteins;hirudin;pharmacokinetics;enzyme-linked immunosorbent assay;partial thromboplastin time; 重组蛋白质类;水蛭素;药物动力学;酶联免疫吸附测定;部分促凝血酶原时间;
- Keywords recombinant protein;E. coli;periplasm secretion;extracellular secretion; 重组蛋白;大肠杆菌;胞周质分泌;胞外分泌;
- The Expression Analysis of Recombinant Protein in Escherichia coli. 大肠杆菌中表达外源重组蛋白的研究。
- Cloning, expression and purification of recombinant protein. 运用常规分子生物学与生化技术克隆表达与纯化蛋白质。
- Production of recombinant proteins in plants offers economic adantages. 在植物中生产重组蛋白拥有经济上的优势。
- It turns that most of the recombinant protein were inclusion body. 对重组蛋白的细胞内定位分析发现蛋白几乎全部以包涵体形式存在。
- Inactive inclusion bodies are often formed during overexpression of recombinant proteins in prokaryotes. 用基因工程技术在大肠杆菌高水平表达重组蛋白时，通常形成无生物活性的包涵体。
- However, the recovery of recombinant proteins hinges largely on the ability to effectively induce cell lysis. 然而，重组蛋白的提取有赖于诱导细胞溶解的能力。
- The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. 摘要昆虫杆状病毒表达载体系统已广泛应用于表达重组蛋白。
- The recombinant proteins were expressed successfully and the purity was over 90% after purified by affinity chromatography. 经表达和纯化获得了纯度达90%25以上的融合蛋白;
- Experience with optimizing the expression of IgGs and other recombinant proteins in industrially relevant platform cell types. 具有采用哺乳动物细胞培养用于生产重组蛋白和抗体的工业生产经验。
- Keywords recombinant;immunotoxin;fusion protein; 重组;免疫毒素;融合蛋白;
- Overexpression of recombinant proteins in \%E.coli\% often results in formation of insoluble, inactive inclusion bodies. 外源基因在大肠杆菌中高水平表达时,通常会形成无活性的蛋白聚集体即包涵体。
- Abstract: The baculovirus expression system has been used extensively for the expression of recombinant proteins in insect cells. 摘要: 昆虫杆状病毒表达载体系统已广泛应用于表达重组蛋白。
- Method Applying the HEV recombinant proteins with known sequences as the coated antigens, the serum samples with known background were tested by ELISA. 方法:用已知序列的HEV基因工程表达蛋白作为包被抗原,对已知血清标本进行酶联免疫吸附试验测定。
- Then, incubate the purified recombinant proteins with human primary hepatocyte membrane proteins, which contain the cellular receptor of HBV. 再将重组蛋白与人类原始肝细胞的细胞膜蛋白进行结合实验，人类原始肝细胞确定可感染HBV并含有HBV细胞受器。
- Industrial experience in developing mammalian cell culture processes for manufacturing of recombinant proteins and antibodies. 具有采用哺乳动物细胞培养用于生产重组蛋白和抗体的工业生产经验。
- The Kluyveromyces lactis expression system is being used successfully for the production of various heterologous recombinant proteins. 乳酸克鲁维酵母已成功地应用于多种异源蛋白的表达生产之中。
- In an earlier study, genotype 1 HE recombinant protein accine was shown to be effectie in nonhuman primates. 在一项较早的研究中，HE基因型1重组蛋白疫苗对非人类灵长目有效。
- One of the more extensively used systems for the expression and purification of recombinant proteins is the Glutathione-S-Transferase(GST)Gene Fusion System. 一个比较广泛应用于重组蛋白表达和纯化的系统是:谷胱甘酞转移酶基因融合表达系统.
